Toxic effects of NH4+-N on embryonic development of Bufo gargarizans and Rana chensinensis

- Nitrogen fertilizers can damage ecosystems health while benefiting crop production.
- The effects of NH4+-N on two Chinese native amphibian species were compared.
- B. gargarizans and R. chensinensis embryos responded differently to NH4+-N.
- NH4+-N exposure may increase abnormality and inhibit embryo hatching and development.
- It has implication for fertilizer management and environmental standard for NH4+-N.

Although nitrogen fertilizer is commonly used worldwide, little information is currently available about NH4+-N toxicity on amphibians. This study determined the acute and chronic toxic effects of NH4+-N on two native Chinese amphibian species (Bufo gargarizans and Rana chensinensis), and compared the negative sensitivity of different embryos to NH4+-N. Static renewal aqueous exposures were performed using B. gargarizans and R. chensinensis embryos at Gosner stage 2 over 96 h. In terms of 96 h-LC50, B. gargarizans and R. chensinensis embryos had significantly different responses to NH4+-N, and the latter was more sensitive to NH4+-N than the former. In the chronic toxicity test, exposure to 10 mg L−1NH4+-N or higher significantly decreased the hatching rate of embryos in both species. Significant increases in the abnormality rate of embryos at 50 mg L−1NH4+-N or higher were observed and morphological abnormalities were characterized by axial flexures, yolk sac edema, and hyperplasia in both species. Additionally, the total length of embryos decreased in a dose-dependent manner after exposure to NH4+-N. The results indicate that NH4+-N exposure can increase abnormality and inhibit the hatching and development of embryos in B. gargarizans and R. chensinensis.