Mortality and movement of Cryptolestes ferrugineus and Rhyzopertha dominica in response to cooling in 300-kg grain bulks

- During the 3 weeks of cooling, grain temperatures went from 38 to −5 °C.
- R. dominica were mainly in the top of grain, C. ferrugineus were throughout grain.
- R. dominica adults were more susceptible to cold than C. ferrugineus.
- There was some survival of immatures of both species after 3 weeks cooling.

We examined the effect of low temperatures on insect population density and mortality of adult and immature stages of Cryptolestes ferrugineus and Rhyzopertha dominica in three barrels holding 300 kg of wheat each. Barrels were infested with 2 insects/species/kg and left to develop at 30 °C for 5 months. Temperature of the grain mass before cooling was 38 °C in the centre to a low of 28 °C on the periphery. On 11 February the building door was opened to allow for cooling of the grain. During the 3 weeks of cooling, temperatures in the centre reached as low as −5 °C. Segregated (11 segments) grain trier samples were collected from three locations in each barrel, from February until March 2013 (6 dates), sieved, and the number of live and dead adults counted. Sieved grain was held for 5 weeks 30 °C and emerged adults counted to give an estimation of immature stages within the grain at the time of sampling. Rhyzopertha dominica adults were found mainly in the top 40 cm of the barrel, and there was little movement to the centre as the grain mass cooled. The initial distribution of C. ferrugineus adults was more complex; in two barrels adults were mostly in the centre of the grain mass, while in the other barrel, more adults were found on the periphery. When the grain mass cooled, C. ferrugineus adults in all barrels were found mostly in the centre. On day 0, the average number of live adults of C. ferrugineus and R. dominica were 232 ± 56 and 414 ± 48 insects/100 g, respectively. Rhyzopertha dominica adults were more susceptible to low temperatures than C. ferrugineus. The density of immatures of C. ferrugineus and R. dominica were similar at 0 d, there was a decline over the 21 d, but there was some survival at day 21.