Effects of selenite on green microalga Haematococcus pluvialis: Bioaccumulation of selenium and enhancement of astaxanthin production

Algae are at a low trophic level and play a crucial role in aquatic food webs. They can uptake and accumulate the trace element selenium (Se), which can be either essential or toxic to algal growth depending on the dosage and species. Se toxicity and algae resistance varied across different organisms. In order to investigate the effects of Se on the unicellular green alga Haematococcus pluvialis, an important industrial resource for natural astaxanthin, the algal growth rate, chlorophyll content, and fluorescence parameters were derived from experimental treatment with different concentrations of selenite. The results showed that the EC50 for the algal growth rate was 24 mg/L, and that a low dosage of selenite (3 mg/L) may not hinder H. pluvialis cell growth, but selenite at levels higher than 13 mg/L do restrain cell growth. Bioaccumulation experiments showed that H. pluvialis accumulated up to 646 μg/g total Se and 380 μg/g organic Se, dry weight. However, treatment with high concentrations of selenite significantly increased intracellular hydrogen peroxide levels, antioxidant enzyme activity, and the production of astaxanthin, suggesting that Se bioaccumulation might be toxic to H. pluvialis.