کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5767175 1628383 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A survey on the Aflatoxin M1 occurrence and seasonal variation in buffalo and cow milk from Southern Italy
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
A survey on the Aflatoxin M1 occurrence and seasonal variation in buffalo and cow milk from Southern Italy
چکیده انگلیسی


- Analysis of aflatoxin M1 in samples of cow and buffalo raw milk from was performed.
- Only a cow milk sample exceeded the maximum limit fixed by the European Regulation.
- Contamination was significantly higher in cold than in warm season.

This study evaluates the aflatoxin M1 (AFM1) contamination in 804 samples of raw milk from cow and buffalo, collected randomly in Campania and Calabria regions of Southern Italy over a two years period.The competitive enzyme linked immunosorbent assay (ELISA) method was used to analyze AFM1 in the samples. AFM1 levels result above the CCβ value of 0.004 μg/kg in 51 (12.3%) cow milk samples and in 28 (7.2%) buffalo milk samples. Positive results from screening analysis were confirmed by high performance liquid chromatography with fluorimetric detection (HPLC-FLD) after a procedure of centrifugation, extraction and immunoaffinity column clean-up of milk. Only one cow milk sample exceeded the maximum limit (0.05 μg/kg) set by the European Regulation.The occurrence of AFM1 contamination was significantly (p < 0.05) higher in cold season, particularly fall, than in warm season, principally spring.Our results indicate that feedstuff used in the buffalo and cow farms were not highly contaminated with aflatoxins, determining a good quality of the analyzed milk. Therefore, the AFM1 contamination of the milk does not represent a serious public health problem in both regions in Southern Italy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 81, November 2017, Pages 30-33
نویسندگان
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