کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5767204 1628379 2018 5 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Development of a fast duplex real-time PCR assay for simultaneous detection of chicken and pigeon in raw and heat-treated meats
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI
فقط 3 هزار تومان
سفارش ترجمه تخصصی
با تضمین قیمت و کیفیت
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Development of a fast duplex real-time PCR assay for simultaneous detection of chicken and pigeon in raw and heat-treated meats
چکیده انگلیسی


- Fast duplex real-time PCR was developed for the detection of chicken and pigeon.
- The detection limits were 0.01% for pigeon or chicken in meat mixtures.
- The method simultaneously identified the two meats in under 40 min.
- The applicability of this assay was tested using raw and heat-treated meat samples.

We developed a fast duplex real-time polymerase chain reaction assay based on TaqMan® probes for the simultaneous identification of chicken and pigeon meat in under 40 min. The primer sets used for both pigeon and chicken targeted the mitochondrial cytochrome b gene. To obtain simultaneous fluorescent signals, FAM and HEX probes were used to label the pigeon- and chicken-specific probes, respectively. This method specifically amplified chicken and pigeon DNA without any cross-reactivity to 20 animal species. The absolute detection limit was 0.1 pg of DNA extracted from both chicken and pigeon meat. Using DNA extracted from either raw or heat-treated meat, as little as 0.01% pigeon DNA was detectible in chicken DNA, as well as the reverse. This assay might serve as a rapid, sensitive, and specific detection method for the identification of pigeon and chicken meats in both raw- and cooked-meat products.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 85, March 2018, Pages 1-5
نویسندگان
, ,
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI
فقط 3 هزار تومان
سفارش ترجمه تخصصی
با تضمین قیمت و کیفیت