|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5767204||1413191||2018||5 صفحه PDF||ندارد||دانلود کنید|
â¢Fast duplex real-time PCR was developed for the detection of chicken and pigeon.â¢The detection limits were 0.01% for pigeon or chicken in meat mixtures.â¢The method simultaneously identified the two meats in under 40Â min.â¢The applicability of this assay was tested using raw and heat-treated meat samples.
We developed a fast duplex real-time polymerase chain reaction assay based on TaqManÂ® probes for the simultaneous identification of chicken and pigeon meat in under 40Â min. The primer sets used for both pigeon and chicken targeted the mitochondrial cytochrome b gene. To obtain simultaneous fluorescent signals, FAM and HEX probes were used to label the pigeon- and chicken-specific probes, respectively. This method specifically amplified chicken and pigeon DNA without any cross-reactivity to 20 animal species. The absolute detection limit was 0.1Â pg of DNA extracted from both chicken and pigeon meat. Using DNA extracted from either raw or heat-treated meat, as little as 0.01% pigeon DNA was detectible in chicken DNA, as well as the reverse. This assay might serve as a rapid, sensitive, and specific detection method for the identification of pigeon and chicken meats in both raw- and cooked-meat products.
Journal: Food Control - Volume 85, March 2018, Pages 1-5