کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5767511 1628386 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Diversity of Salmonella isolates and their distribution in a pig slaughterhouse in Huaian, China
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Diversity of Salmonella isolates and their distribution in a pig slaughterhouse in Huaian, China
چکیده انگلیسی


- We demonstrate significant diversity of Salmonella isolate distribution in a pig slaughterhouse.
- Contamination of Salmonella frequently occurs between carcass and other areas.
- We identified dynamic changes in Salmonella in successive slaughtering processes.

In order to ascertain the contamination status of Salmonella from carcass, equipment, environment and the intestinal contents in a pig slaughterhouse in Huaian, China, we investigated the prevalence and characteristics of Salmonella isolates in one slaughterhouse. A total of 636 samples were collected; Salmonella was laboratory-confirmed from 154/441 (34.9%) carcass swab-samples, 13/57 (22.8%) pieces of equipment, 30/86 (34.9%) environmental samples and the intestinal contents of 19/52 (36.5%) animals. Salmonella was found to be at its highest and lowest frequency during splitting (55.9%) and flaming (17.2%) processes, respectively. All Salmonella isolates were characterized by serotyping, multilocus sequence typing (MLST) and 69 S. Derby isolates were analyzed using pulsed-field gel electrophoresis (PFGE). Four serovars (S. Derby, S. Rissen, S. Typhimurium, and S. London) were shared from the four sources and predominant serovars were S. Derby and S. Rissen. MLST results indicated that ten different STs were distinguished, of which ST40 was identified as the most prominent ST. Moreover, ST40, ST469, ST34 and ST155 comprised Salmonella isolates obtained from carcass, equipment, the slaughterhouse environment and intestinal contents. PFGE results showed that 33 pulsotypes (PF1-PF33) were identified. PF21, PF25 and PF29 suggested that many isolates from carcass-swab samples at different slaughterhouse process points were of the same pulsotype which also partly comprised isolates from equipment, the environment and intestinal contents. The present study provides an insight into Salmonella distribution in a pig slaughterhouse and revealed that cross-contamination occurs frequently at different sites, which provides data support for proposing some interventions to control Salmonella.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 78, August 2017, Pages 238-246
نویسندگان
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