کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5767700 | 1413202 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Graphene oxide (GO) is proposed to be able to quench the fluorescence signal.
- β-lactamase aptamer was used to replace the traditional antibody.
- Based on aptamer assembled onto GO, a new, sensitive and direct determination of β-lactamase was established.
- The established method was verified by ELISA.
Fluorescent sensor assay (FSA) was developed by using a fluorescein-labeled aptamer assembled onto Graphene oxide (GO) in order to determine β-lactamase in milk. Under optimal conditions, FSA indicated a detection range from 1 to 46 U/mL with a limit of detection (LOD) of 0.5 U/mL (R2 = 0.999, n = 3). In addition, commercial milk samples tainted with β-lactamase were detected by the established FSA with a recovery rate between 96.04 and 119.67%. Additionally, the reliability and sensitivity of FSA was validated by enzyme-linked immunoassay (ELISA) with a high correlation of 0.993. Thus, these data, combined with the ease and speed of the assay, suggest that the developed FSA may represent a promising method for monitoring β-lactamase contamination in milk.
Journal: Food Control - Volume 73, Part B, March 2017, Pages 726-733