|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5769461||1413240||2018||7 صفحه PDF||سفارش دهید||دانلود کنید|
- We examined plant growth, NO3â-N assimilation enzyme and transaminase activities, and endogenous GABA concentration in muskmelon seedlings.
- Foliar spraying with 50Â mM of GABA regulates the NO3â-N absorption and assimilation and alleviates Ca(NO3)2-induced growth inhibition.
- Exogenous GABA application improves salt tolerance of muskmelon.
The potential of exogenous Î³-aminobutyric acid (GABA) application to improve the NO3â-N absorption and assimilation in Ca(NO3)2-treated muskmelon seedlings was investigated in 'Yipintianxia 208', a salt-sensitive melon cultivar grown in a deep-flow hydroponic system. Plants were treated under control or 80Â mM Ca(NO3)2 stress conditions with or without foliar spraying 50Â mM GABA. We found that under Ca(NO3)2 stress, the activities of nitrate reductase (NR), glutamate synthetase (GS), and glutamate amino-transferase (GOGAT) in muskmelon seedlings were significantly reduced, while the activities of glutamate dehydrogenase (GDH), glutamate oxaloacetate transaminase (GOT), and glutamate pyruvate aminotransferase (GPT) were enhanced. These changes lead to a decrease in the content of NO3â-N and an increase in that of NH4+-N in muskmelon roots and leaves, which further severely inhibited the plant growth. Exogenous GABA application effectively improved the absorption of NO3â-N in muskmelon roots and leaves under Ca(NO3)2 stress and the NH4+-N assimilation by enhancing the activities of nitrogen assimilation enzymes (NR, GS, and GOGAT) in muskmelon seedlings. Exogenous GABA also reduced the NH4+ release by limiting the GDH deamination, thus further alleviating the ammonia toxication induced by Ca(NO3)2 stress. Our results suggest that exogenous GABA applications can relieve the nitrogen metabolic disorders caused by Ca(NO3)2 stress and eventually promote plant growth. In addition, Exogenous GABA regulated the transaminase (GOT and GPT) activities, maintaining the balance of the amino acid metabolism. Furthermore, the accumulation of the functioning as a nitrogen source endogenous GABA was promoted, eventually improving the resistance of muskmelon seedlings to Ca(NO3)2 stress.
Journal: Scientia Horticulturae - Volume 227, 3 January 2018, Pages 117-123