کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5898700 1568794 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intravenous vs intraperitoneal transplantation of umbilical cord mesenchymal stem cells from Wharton's jelly in the treatment of streptozotocin-induced diabetic rats
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Intravenous vs intraperitoneal transplantation of umbilical cord mesenchymal stem cells from Wharton's jelly in the treatment of streptozotocin-induced diabetic rats
چکیده انگلیسی


- Umbilical cord mesenchymal stem cells have the potential to differentiate into pancreatic beta cells in vitro and in vivo.
- Effective administration route for mesenchymal stem cells transplantation is proposed.
- Tail vain injection of mesenchymal stem cells alleviates hyperglycemia effectively in the diabetic rats whereas intraperitoneal injection does not.
- Diabetic rats treated with tail vein injection shows markedly increase in the area and diameter of the pancreatic islets.

AimTo evaluate the efficiency of mesenchymal stem cells isolated from Wharton's jelly (WJ-MSCs) through either the intravenous or intraperitoneal transplantations into streptozotocin (STZ)-induced diabetic rats as a therapy for type 1 diabetes mellitus (T1DM).MethodologyA rat model with STZ induction was established and the rats were divided into 3 groups: a tail vein injection group, an intraperitoneal injection group and a STZ control group. Following transplantation, blood glucose levels were monitored weekly then the pancreatic tissues were collected to examine the pancreatic islets by histopathology and morphometric studies.ResultsIntravenous transplantation of WJ-MSCs ameliorated hyperglycemia at day 7 after transplantation, with sustained decreased fasting blood glucose (FBG) levels until day 56. Further, these cells ameliorated at least partially the damage induced by STZ in the pancreas and produced a similar morphology to normal islets. On the contrary, intraperitoneal transplantation of WJ-MSCs failed to maintain normoglycemia or ameliorate the damaged pancreas in STZ-injected rats.ConclusionThese findings conclude that the intravenous administration method was effective in transplanting WJ-MSCs for the treatment of T1DM, whereas the intraperitoneal transplantation showed no therapeutic effect in our animal experiments.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diabetes Research and Clinical Practice - Volume 121, November 2016, Pages 102-111
نویسندگان
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