کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5900361 | 1155644 | 2011 | 10 صفحه PDF | دانلود رایگان |
AimIn this study we investigated if the â786TÂ >Â C, the VNTR intron 4 a/b and the 894GÂ >Â T (Glu298Asp) polymorphisms in the eNOS gene were associated with renal disease in 617 type 2 diabetic Caucasian-Brazilians. These polymorphisms were also examined in 100 Caucasian healthy blood donors.MethodsGenotyping of eNOS polymorphisms was performed by PCR or PCR-RFLP and haplotype frequencies were estimated using a Bayesian method. Logistic regression analysis was done to test for association of eNOS polymorphisms with susceptibility to renal involvement (microalbuminuria, macroalbuminuria or end-stage renal disease). This analysis was carried out assuming three diferent genetic models for the minor allele, adjusting for possible effect modifiers.ResultsGenotype and allele frequencies in patients with renal disease were not significantly different from those of patients with normoalbuminuria and healthy blood donors for all eNOS polymorphisms. Likewise, there were no differences in haplotype frequencies among healthy blood donors and type 2 diabetic patients with or without renal involvement (PÂ >Â 0.05 for all comparisons).ConclusionNo associations between the â786TÂ >Â C, the VNTR intron 4 a/b and the 894GÂ >Â T (Glu298Asp) polymorphisms in the eNOS gene and renal disease were observed in type 2 diabetic Caucasian-Brazilians.
Journal: Diabetes Research and Clinical Practice - Volume 91, Issue 3, March 2011, Pages 353-362