کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905085 | 1159829 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of apoptosis between adult worms of Schistosoma japonicum from susceptible (BALB/c mice) and less-susceptible (Wistar rats) hosts
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کلمات کلیدی
IAPCASP9CASP7apoptotic protease-activating factorS. japonicumAPAFBcl-2 homologous antagonist killerPZQCYCTNFRCASP3FCMAPIPBSBcl-2RT-PCRataxia telangiectasia mutated - Ataxia telangiectasia جهش یافته استcIAP - ciapAIF - آیفونTem - این استBax - باکسTUNEL - تونلATM - خودپردازcytochrome c - سیتوکروم سیSchistosoma japonicum - شیستوزوما ژاپونیکومapoptosis-inducing factor - عامل القاء آپوپتوزPhosphate buffered saline - فسفات بافر شورFlow cytometry - فلوسیتومتریB-cell lymphoma-2 - لنفوم سلول B-2Apoptosis inhibitor - مهار کننده آپوپتوزTransmission electron microscopy - میکروسکوپ الکترونی عبوریNADH - نادانnicotinamide adenine dinucleotide dehydrogenase - نیکوتین آمید آدنین دینکلوتید دهیدروژنازreal-time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPraziquantel - پرازیکوانتلBCL2-associated X protein - پروتئین X مرتبط با BCL2Propidium iodide - پروتئین یدیدBAK - پشتcaspase 3 - کاسپاز 3Caspase 7 - کاسپاز 7Caspase 9 - کاسپاز 9tumor necrosis factor receptor - گیرنده فاکتور نکروز تومور
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Schistosomiasis remains a serious public health concern in China. BALB/c mice are susceptible to Schistosoma japonicum infection, whereas the Wistar rats are less susceptible. Apoptosis phenomenon was observed in 42Â d adult worms of S. japonicum from both rats and mice at the morphologic, DNA, cellular, and gene levels by transmission electron microscopy (TEM), fluorometric terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) analysis, fluorescein isothiocyanate-annexin-V/propidium iodide staining flow cytometry (FCM) analysis, and real-time PCR. The results showed that the apoptotic state in worms from two different susceptible hosts was diverse. Several classical hallmarks of apoptosis, including cell shrinkage, chromatin condensation and lunate marginalization, splitting of the nucleoli, nuclear shrinkage and apoptotic body formation were observed by TEM. TUNEL analysis showed that there were much more apoptosis spots in adult worms from rats than those from mice. Statistical analysis revealed that the degree of apoptosis and percentage of necrotic cells in adult worms from Wistar rats were significantly greater (PÂ <Â 0.01) than those from BALB/c mice by flow cytometry. A total of 15 apoptosis-associated genes including the major components of an intrinsic cell-death pathway were identified from S. japonicum in this study, suggested that a similar apoptosis pathway might occur in S. japonicum. Real-time PCR analyses revealed that the expression levels of most of the tested apoptosis-associated genes, except CASP7, were significantly higher or at the similar level in adult worms from Wistar rats, as compared to those from BALB/c mice. The results obtained in this study collectively demonstrated that differential development of adult S. japonicum in less-susceptible rats and susceptible mice was significantly associated with apoptosis in the worm, and provided valuable information to guide further investigations of the mechanisms governing apoptosis and host interactions in schistosome infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 592, Issue 1, 30 October 2016, Pages 71-77
Journal: Gene - Volume 592, Issue 1, 30 October 2016, Pages 71-77
نویسندگان
Tao Wang, Xiaoyong Guo, Yang Hong, Hongxiao Han, Xiaodan Cao, Yanhui Han, Min Zhang, Miaoli Wu, Zhiqiang Fu, Ke Lu, Hao Li, Zhixin Zhao, Jiaojiao Lin,