کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5915348 1163282 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus
چکیده انگلیسی


- Eleven candidate reference genes were evaluated in Haemonchus contortus adults.
- Comparisons were made between sexes, isolates and anthelmintic treatments.
- Gpd, far and ama are recommended for expression studies in adult H. contortus.

The aim of this work was to identify reliable reference genes for expression studies in adult Haemonchus contortus. Eleven candidate genes were identified and the stability of their expression was assessed in adult males and females of two genetically divergent H. contortus isolates: drug-susceptible (ISE) and multi-drug-resistant (WR). Five genes with the most stable expression patterns were further assessed for suitability as reference genes in anthelmintic-treated H. contortus adults versus non-treated controls. We identified important differences in the expression of a number of candidate genes in anthelmintic-treated samples, confirming the need for careful validation of control genes for such experiments. We propose the use of multiple reference genes for expression studies in this species and found gpd, ama and far most suitable for adult H. contortus.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 201, Issue 2, June 2015, Pages 123-127
نویسندگان
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