کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5915893 1163338 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short communicationThe role of local secondary structure in the function of the trans-splicing motif of Brugia malayi
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Short communicationThe role of local secondary structure in the function of the trans-splicing motif of Brugia malayi
چکیده انگلیسی

A 7-nt motif (the trans-splicing motif or TSM) was previously shown to be necessary and sufficient to direct trans-splicing of transgenic mRNAs in transgenic Brugia malayi embryos. Insertion of the TSM into two genes lacking a TSM homologue resulted in trans-splicing of transgenic mRNAs from one transgene but not the other, suggesting that local sequence context might affect TSM function. To test this hypothesis, constructs inserting the TSM into different positions of two B. malayi genes were tested for their ability to support trans-splicing of transgenic mRNAs. Transgenic mRNAs derived from constructs in which the insertion of the TSM did not result in a perturbation of the local predicted secondary structure were trans-spliced, while those in which the TSM perturbed the local secondary structure were not. These data suggest that local secondary structure plays a role in the ability of the TSM to direct trans-splicing.

Local pre-mRNA secondary structure appears to play a role in the ability of the Brugia malayi trans-splicing motif to support trans-splicing of transgenic mRNAs.38

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 169, Issue 2, February 2010, Pages 115-119
نویسندگان
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