کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133041 | 1593452 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A suicidal DNA vaccine expressing the fusion protein of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in mice
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کلمات کلیدی
PPROIETCID50World Organization for Animal HealthIFASFVPPRVGMTSuicidal DNA vaccinekDaHRPCPEDMEMDABVNA3,3′-diaminobenzidine tetrahydrochloride - 3،3'-diaminobenzidine tetrahydrochlorideDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoPeste des petits ruminants virus - Peste des petits معتادین ویروسPeste des petits ruminants - Peste des petits یادآورانindirect immunofluorescence assay - آزمایش ایمنی فلوئورسانس غیر مستقیمVirus neutralizing antibody - آنتی بادی ضد ویروس ویروسCytopathic effect - اثر سیتوپاتیکbaby hamster kidney - بچه هامستر کلیهdifferentiating infected from vaccinated animals - تمایز حیوانات واکسینه شده را آلوده می کندDIVA - دیواVero cell - سلول Veroafrican green monkey kidney cells - سلول های کلیه میمون سبز آفریقاییstimulation index - شاخص تحریکGeometric mean titer - میانگین میانگین هندسیhemagglutinin - هماگلوتینینSemliki Forest virus - ویروس Semliki جنگلImmune response - پاسخ یا واکنش ایمنیHorseradish peroxidase - پراکسیداز هوررادیشF protein - پروتئین FFusion protein - پروتئین فیوژن یا پروتئین همجوشیKilo Dalton - کیلو دالتون
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Peste des petits ruminants (PPR), a highly contagious disease induced by PPR virus (PPRV), affects sheep and goats. PPRV fusion (F) protein is important for the induction of immune responses against PPRV. We constructed a Semliki Forest virus (SFV) replicon-vectored DNA vaccine (“suicidal DNA vaccine”) and evaluated its immunogenicity in BALB/c mice. The F gene of PPRV was cloned and inserted into the SFV replicon-based vector pSCA1. The antigenicity of the resultant plasmid pSCA1/F was identified by indirect immunofluorescence and western blotting. BALB/c mice were then intramuscularly injected with pSCA1/F three times at 14-d intervals. Specific antibodies and virus-neutralizing antibodies against PPRV were quantified by indirect ELISA and microneutralization tests, respectively. Cell-mediated immune responses were examined by cytokine and lymphocyte proliferation assays. The pSCA1/F expressed F protein in vitro and induced specific and neutralizing antibody production, and lymphocyte proliferation in mice. Mice vaccinated with pSCA1/F had increased IL-2 and IL-10 levels after 24-h post first immunization. IFN-γ and TNF-α levels increased from that time point and gradually decreased thereafter. Thus, the Semliki Forest virus replicon-vectored DNA vaccine expressing the F protein of PPRV induced both humoral and cell-mediated immune responses in mice. This could be considered as a novel strategy for vaccine development against PPR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 225, 1 December 2015, Pages 35-40
Journal: Journal of Virological Methods - Volume 225, 1 December 2015, Pages 35-40
نویسندگان
Yong Wang, Xiaolin Yue, Hongyan Jin, Guangqing Liu, Ling Pan, Guijun Wang, Hao Guo, Gang Li, Yongdong Li,