کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6263448 | 1613896 | 2014 | 6 صفحه PDF | دانلود رایگان |
- There is no report of in vivo leukocyte behavior in brain microvessels after ischemia.
- Effects of cilostazol on platelet in ischemic brain microvessels were studied.
- Cilostazol inhibits leukocyte-endothelial interactions after brain transient ischemia.
Leukocyte behavior in the cerebral microvasculature following vessel occlusion has not been fully elucidated. The purpose of this study was to investigate the effects of cilostazol on leukocyte behavior (rolling and adhesion) in murine cerebral microvessels following transient bilateral carotid artery occlusion using intravital fluorescence microscopy. Four groups of mice were assigned: a sham group (n=16); an ischemia (induced by 15-min occlusion of bilateral common carotid arteries) and reperfusion (I/R) group (n=13); I/R+cilostazol (I/R+CZ3Â mg/kg) group (I/R after oral administration of cilostazol at 3Â mg/kg) (n=8); and I/R+cilostazol (I/R+CZ30Â mg/kg) group (I/R after oral administration of cilostazol at 30Â mg/kg) (n=12). Leukocytes labeled with 0.05% acridine orange were administered intravenously and their behavior was investigated at 3 and 6Â h after reperfusion. Numbers of rolling or adherent leukocytes were expressed as the count per square millimeter per 30Â s. Numbers of rolling and adherent leukocytes at 3 and 6Â h after reperfusion were significantly higher in the I/R group than in the sham or I/R+CZ30Â mg/kg groups in both pial veins (P<0.05) and pial arteries (P<0.05). Cilostazol (30Â mg/kg) inhibited leukocyte-endothelial interactions following cerebral ischemia and reperfusion.
Journal: Brain Research - Volume 1543, 16 January 2014, Pages 173-178