کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
639950 1456958 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An approach based upon the consecutive separation and the economical two-phase solvent system preparation using UNIFAC mathematical model for increasing the yield of high-speed counter-current chromatography
ترجمه فارسی عنوان
یک روش برای جداسازی متوالی و آماده سازی سیستم حلال دو فازی اقتصادی با استفاده از مدل ریاضی UNIFAC برای افزایش عملکرد کروماتوگرافی با سرعت بالا جریان ناهمسو
کلمات کلیدی
سرعت بالا کروماتوگرافی مخالف؛ جدایی انتقال پی در پی؛ مدل ریاضی UNIFAC؛ کاج massoniana L؛ Tiliroside
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
چکیده انگلیسی

A HSCCC method combined with the consecutive injection and the economical two-phase solvent system preparation using UNIFAC mathematical model was successfully developed for increasing the yield of target compound. HSCCC was performed with a two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (3:5:3:5, v/v) at a flow rate of 6 mL/min. The components of the upper and lower phases were calculated according to UNIFAC mathematical model to prepare the stationary and mobile phases, separately. Only 300 mL stationary phase was filled into the column, and the crude sample (200 mg, ten times) was consecutive loaded into the column at the given interval time and eluted using the individually prepared mobile phase without reestablishing hydrodynamic equilibrium. After recrystallized, 168 mg tiliroside with the purity of 95.8% was obtained from 2 g crude sample of Pinus massoniana L. The structure was identified by 1H NMR and 13C NMR. The present method not only avoided the time consuming of exchanging the new solvent system and reestablishing hydrodynamic equilibrium, but also coped with a large amount of organic solvent waste.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 162, 13 April 2016, Pages 142–147
نویسندگان
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