کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6402007 1330885 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Digestion of protein and protein gels in simulated gastric environment
ترجمه فارسی عنوان
هضم ژل پروتئین و پروتئین در محیط شبیه سازی معده
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- Protein gels were used as models to study the protein digestion of solid food.
- Microstructure affects the gastric digestion of protein gels.
- Different digestion kinetics were observed between protein gels and solutions.
- A descriptive model is proposed regarding the enzymatic hydrolysis of protein gels.

Despite the increasing attention to food digestion research, food scientists still need to better understand the underlying mechanisms of digestion. Most in vitro studies on protein digestion are based on experiments with protein solutions. In this study, the digestion of egg white protein and whey protein isolate in solution and in gels was investigated using simulated gastric conditions. The digestion process was followed via the dry matter loss, degree of hydrolysis and peptide distribution. We showed that the performance of pepsin is an important factor in protein digestion, and hydrodynamic force effectively disintegrated the gel particles and enhance the hydrolysis of protein. The gel microstructure had shown to be a hindrance for the digestion of protein. However, the hindrance is not simply slowing down the hydrolysis, but also altering the apparent enzyme kinetics to some extent: while the dissolved proteins were hydrolysed through a 'zipper' type mechanism, the gels showed a slower 'one-by-one' mechanism. Overall, we believe that the digestion of the protein gels is influenced by the microstructure of food matrices, caused by the immobilisation of the substrate in the network, and the steric hindrance in the diffusive ingression of pepsin and egression of peptides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 63, Issue 1, September 2015, Pages 161-168
نویسندگان
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