Invited paperA new pyrene-based fluorescent probe with large Stokes shift for detecting hydrogen peroxide in aqueous solution and living cells

- Pyrene-based fluorophore as a novel fluorescent dye for developing a fluorescent probe Py-Boe with large Stokes shift in various solutions.
- The probe Py-Boe is capable of concurrently high sensitive and selective detecting H2O2 in aqueous solution.
- The probe Py-Boe is capable of detecting exogenous and endogenous H2O2 in mitochondria of living RAW 264.7 cells

Hydrogen peroxide (H2O2) plays an important role in signal transduction and therapy of serious diseases. In this work, we have developed a new pyrene-based fluorescent probe Py-Boe, which possesses large Stokes shift and achieves detection of H2O2 in aqueous solution. The probe Py-Boe exhibits excellent photostability, high sensitive and selectivity. Taking advantage of these properties, the probe Py-Boe achieves succussfully imaging of exogenous and endogenous H2O2 in living RAW 264.7 cells. We expect that this dye with large Stokes shift may open an avenue to construct novel fluorescent probe with large Stokes shift for biological application.

Herein, we have developed a new pyrene-based fluorescent probe Py-Boe with large Stokes shift and achieved high sensitive detection of H2O2 in aqueous solution. Furthermore, the probe Py-Boe is suitable for detecting exogenous and endogenous H2O2 in living RAW 264.7 cells.131