Trithiane silver-nanoparticles-decorated polyaniline nanofibers as sensing element for electrochemical determination of Adenine and Guanine in DNA

- Synthesis of Trithiane-Ag-nanoparticles, decorating polyaniline nanofibers for the first time via simple chemical reactions.
- Decorated nanofibers for a novel electrochemical probe for Adenine and Guanine
- Simultaneous or individual determination of Adenine and Guanine in samples
- The sensor provides a simple, sensitive, durable and precise tool for Adenine and Guanine.
- This sensor shows separate and well-defined peaks for accurately determination of Adenine and Guanine in DNA.

Here, we report the synthesis of 1,3,5-Trithiane-Ag-nanoparticles decorated polyaniline nanofibers (TAN-AgNP-PANF) and describe its benefit in the sensing element of an electrochemical sensor for simultaneous detection of Adenine (A) and Guanine (G). We characterized the electrode composition with field emission-scanning electron microscopy (FE-SEM), atomic force microscopy (AFM) and Fourier-transform infrared spectroscopy (FT-IR). The electrocatalytic oxidation of A and G on the electrode sensor was explored by differential pulse voltammetry (DPV) and cyclic voltammetry (CV). This sensor shows separate and well-defined peaks for A and G, by which one can determine these biological bases individually or simultaneously. A linear relationship is figured out between the peak current and the analytes' concentrations on range 1.0 to 200 and 0.9 to 140 μM, for simultaneous determination of A and G, with detection limits of 2.8 and 3.0 μM, respectively. Furthermore, individually determination is resulted in two linear concentration ranges of 0.08 to 25.0 and 0.01 to 15.0 μM with detection limits of 24.0 and 0.06 μM for A and G, respectively. The sensor demonstrates a good stability and sufficient selectivity over the amino acids and could be used to quantify of A and G in fish sperm DNA sample successfully.

Novel carbon paste electrode based on 1,3,5-Trithiane silver-nanoparticles decorated polyaniline nanofibers for Adenine and Guanine.166