کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6483869 1415977 2018 22 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Grafting glycidyl methacrylate-iminodiacetic acid conjugate to Sepharose FF for fabrication of high-capacity protein cation exchangers
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Grafting glycidyl methacrylate-iminodiacetic acid conjugate to Sepharose FF for fabrication of high-capacity protein cation exchangers
چکیده انگلیسی
This work was designed to acquire novel high-capacity cation exchangers by grafting glycidyl methacrylate (GMA)-iminodiacetic acid (IDA) conjugate (GMA-IDA) to Sepharose FF. Six GMA-IDA-grafted resins of the same grafting density but different chain lengths (ionic capacities, ICs) were synthesized and denoted as FF-Br2-pG-In (n denotes for IC value). Lysozyme adsorption and chromatography were studied to evaluate the cation exchangers. It was found that resins of moderate IC values (FF-Br2-pG-I480 and FF-Br2-pG-I590) afforded lysozyme adsorption capacities higher than 300 mg/mL at 50 and 100 mmol/L NaCl, over three times higher than the commercial resin CM Sepharose FF. Beside, the uptake rate (De/D0) of lysozyme decreased with increasing IC at 50 mmol/L NaCl because of the increased polymer chain length that limited surface diffusion of the bound protein. Protein adsorption capacities of FF-Br2-pG-I480 and FF-Br2-pG-I590 kept higher than that of CM Sepharose FF in the salt concentration range of 0-150 mmol/L, and the uptake rates on the two resins increased with increasing salt concentration. Therefore, the dynamic binding capacities of them were remarkably higher than that of CM Sepharose FF and kept over 70 mg/mL at the salt concentration up to 150 mmol/L. The results demonstrated that GMA-IDA-grafted resins are promising for use as high-capacity protein chromatography.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 138, 15 October 2018, Pages 74-80
نویسندگان
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