کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
679725 1459955 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Continuous xylanase production with Aspergillus nidulans under pyridoxine limitation using a trickle bed reactor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Continuous xylanase production with Aspergillus nidulans under pyridoxine limitation using a trickle bed reactor
چکیده انگلیسی


• Limited growth fungal system used in trickle bed reactor for enzyme production.
• Enzyme production in TBR better than a tray reactor and similar to shake flask.
• Reactor introduced here combines advantages of SSF and SmF enzyme production.

A trickle bed reactor (TBR) with recycle was designed and tested using Aspergillus nidulans with a pyridoxine marker and over-expressing/secreting recombinant client xylanase B (XynB). The pyridoxine marker prevented the fungus from synthesizing its own pyridoxine and fungus was unable to grow when no pyridoxine was present in the medium; however, enzyme production was unaffected. Uncontrolled mycelia growth that led to clogging of the TBR was observed when fungus without a pyridoxine marker was used for XynB production. Using the fungus with pyridoxine marker, the TBR was operated continuously for 18 days and achieved a XynB output of 41 U/ml with an influent and effluent flow rate of 0.5 ml/min and a recycle flow rate of 56 ml/min. Production yields in the TBR were 1.4 times greater than a static tray culture and between 1.1 and 67 times greater than yields for SSF enzyme production stated in the literature.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 188, July 2015, Pages 219–225
نویسندگان
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