Simultaneous single-step immobilization of Candida antarctica lipase B and incorporation of magnetic nanoparticles on poly(urea-urethane) nanoparticles by interfacial miniemulsion polymerization

• CALB was immobilized on magnetic PUU NPs by interfacial polymerization.
• Immobilization of CALB and the support synthesis were conducted simultaneously.
• Increasing the content of CALB increased the particle diameter.
• Immobilized CALB showed higher esters conversion.

In this work Candida antarctica lipase B (E.C: 3.1.1.3), was immobilized on magnetic poly(urea-urethane) nanoparticles (MNPs-PUU) in a single-step during the interfacial miniemulsion polymerization. Transmission electron microscopy images showed the morphology of synthesized magnetic nanoparticles encapsulated in poly(urea-urethane) nanoparticles and fluorescence microscopy images confirmed the enzyme immobilization onto MNPs-PUU. After the immobilization process, the immobilized enzyme on support (MNPs-PUU) was attracted by an external magnetic field and used as biocatalyst for the synthesis of esters ethyl oleate, geranyl propionate and geranyl oleate. Ester conversions above 85% were obtained for all systems based on the free fatty acids contents measured by titration until pH 11.

Figure optionsDownload as PowerPoint slide