کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7068072 1459888 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Continuous aryl alcohol oxidase production under growth-limited conditions using a trickle bed reactor
ترجمه فارسی عنوان
تولید اکسیداز آرویل الکل بصورت مداوم در شرایط محدود رشد با استفاده از یک رآکتور رطوبت
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی
An A. nidulans strain with a pyridoxine marker was used for continuous production of aryl alcohol oxidase (AAO) in a trickle bed reactor (TBR). Modified medium with reduced zinc, no copper, and 5 g/L ascorbic acid that reduced melanin production and increased AAO productivity under growth limited conditions was used. Two air flow rates, 0.11 L/min (0.1 vvm) and 1.1 L/min (1.0 vvm) were tested. More melanin formation and reduced protein productivity were observed with air flow rate of 1.1 L/min. Three random packings were used as support for the fungus inside the TBR column, two of which were hydrophobic and one which was hydrophilic, and three different dilution rates were tested. The use of GEA BCN 030 hydrophobic packing resulted in greater AAO yield and productivity than the other packings. Increasing dilution rates favored melanin formation and citric, lactic and succinic acid accumulation, which decreased AAO yield and productivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 255, May 2018, Pages 149-155
نویسندگان
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