کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7139690 | 1462023 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rolling circle amplification triggered poly adenine-gold nanoparticles production for label-free electrochemical detection of thrombin
ترجمه فارسی عنوان
تقویت دایره نورد باعث تولید نانوذرات پلی آدنین-طلای برای تشخیص الکتروشیمیایی ترومبین بدون برچسب
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کلمات کلیدی
بدون برچسب آدنین، الکتریکی کاتالیزور، ترومبین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
We developed a novel label-free strategy for electrochemical detection thrombin based on rolling circle amplification (RCA) triggering poly adenine production for adsorption gold nanoparticles (AuNPs). In this assay, the aptamer 1 was first immobilized on the gold electrode (GE) surface as the capture probe via poly adenine-Au. Subsequently, the thrombin and the aptamer 2 were deposited on the electrode surface. The aptamer 2 fragment on the sensor surface as a primer hybridized with the RCA template to initiate the RCA process, which generated massive long DNA sequences that contained many adenines. Subsequently, the AuNPs was absorbed on the long-repeated adenines of RCA product, resulting in the multiplication of AuNPs on the electrode surface, which were used for subsequent electrocatalytic reduction of H2O2. In this sensing system, the electrochemical signals with the concentration of thrombin over a range from 0.1â¯pM to 10â¯nM were detected and the detection limit obtained was 35â¯fM, and have high selectivity toward its target protein. In addition, due to the high affinity between poly adenine and GE or AuNPs, the detection can be carried out without any fussy modification process, thus had a promising application in clinical diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 266, 1 August 2018, Pages 9-18
Journal: Sensors and Actuators B: Chemical - Volume 266, 1 August 2018, Pages 9-18
نویسندگان
Taotao Fan, Yan Du, Yao Yao, Jing Wu, Si Meng, Jianjun Luo, Xing Zhang, Dongzhi Yang, Chunyan Wang, Yong Qian, Fenglei Gao,