Ultrasensitive and eco-friendly immunoassays based monoclonal antibody for detection of deoxynivalenol in cereal and feed samples

Ultrasensitive immunoassays, including an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic assay (ICA), were developed based on a monoclonal antibody for the analysis of deoxynivalenol in food and feed samples. With 0.01 M PBS, 20% ethanol-PBS, and 60% ethanol-PBS extraction, which are environmentally safe, the 50% inhibitory concentration (IC50) and limit of detection (LOD) values were 1.83-4.68 μg/kg and 0.241-0.664 μg/kg, respectively, with recovery rates of 87.7%-137% and coefficient variation values of 3.99-9.88% (intra-assay) and 4.17-9.81% (inter-assay) for the ic-ELISA relative to the results obtained by liquid chromatography-tandem mass spectrometry (LC-MS). For the ICA strip, the visual LODs were 10-150 μg/kg, the cut-off values were 50-750 μg/kg, and the calculated LODs were 1.97-46.8 μg/kg, with different sample extraction solutions, and the recovery rates were 66.7%-127%. These methods are sensitive, simple and safe, providing an auxiliary analytical tool for screening the massive samples in markets.