کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7585844 1492042 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of direct competitive biomimetic immunosorbent assay based on quantum dot label for determination of trichlorfon residues in vegetables
ترجمه فارسی عنوان
توسعه تست ایمونوسیورسانت بیوممینتیک مستقیم بر پایه برچسب نقطه کوانتومی برای تعیین ترشکلونف در سبزیجات
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A direct competitive biomimetic immunosorbent assay method based on molecularly imprinted polymer was developed for the determination of trichlorfon. A CdSe/ZnS quantum dot label was used as the marker. The hydrophilic imprinted film was synthesized directly on the surface of a 96-well plate, and characterized by Fourier-transform infrared spectroscopy and thermo-gravimetric analyses. The method exhibited high stability, selectivity, and sensitivity. Under optimal conditions, the limits of detection and sensitivity of the biomimetic immunosorbent assay method were 9.0 μg L−1 and 5.0 mg L−1 (0.1 mg kg−1 and 62.5 mg kg−1 for vegetable sample), respectively. Low cross-reactivity values of 19.2% and 15.6% were obtained for the structural analogues. Spinach and rape samples spiked with trichlorfon were extracted and determined by this method with recoveries ranging from 83.6% to 91.1%. The method was applied for the detection of trichlorfon residues in leek and cucumber samples, and results correlated well with those obtained using GC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 250, 1 June 2018, Pages 134-139
نویسندگان
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