An insight into the determination of trace levels of benzodiazepines in biometric systems: Use of crab shell powder as an environmentally friendly biosorbent

- Determination of trace levels of benzodiazepines
- Crab shell as a green adsorbent has been provided.
- A vortex assisted dispersive solid phase extraction was used.
- Target analytes were detected in biological matrixes included blood, nail, hair and urine samples.
- Response surface methodology (RSM) based on central composite design (CCD) was used for optimization of factors.