کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7615939 1494028 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fast high-throughput screening of angiotensin-converting enzyme insertion/deletion polymorphism by variable programmed electric field strength-based microchip electrophoresis
ترجمه فارسی عنوان
غربالگری سریع پلی مورفیسم قرار دادن / حذف آنژیوتانسین تبدیل آنزیم با استفاده از الکتروفورز ریز مغذی مبتنی بر قدرت الکتریکی متغیر برنامه ریزی شده
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
An insertion (I)/deletion (D) polymorphism in angiotensin-converting enzyme (ACE) has been associated with susceptibility to various diseases in numerous studies. Traditionally, slab gel electrophoresis (SGE) after polymerase chain reaction (PCR) has been used to genotype this ACE I/D polymorphism. In this study, single- and multi-channel microchip electrophoresis (ME) methods based on variable programmed electric field strength (PEFS) (i.e., low constant, high constant, (+)/(−) staircase, and random electric field strengths) were developed for fast high-throughput screening of this specific polymorphism. The optimum PEFS conditions were set as 470 V/cm for 0-9 s, 129 V/cm for 9-13 s, 470 V/cm for 13-13.9 s, 294 V/cm for 13.9-16 s, and 470 V/cm for 16-20 s for single-channel ME, and 615 V/cm for 0-22.5 s, 231 V/cm for 22.5-28.5 s, and 615 V/cm for 28.5-40 s for multi-channel ME, respectively. In the multi-channel PEFS-ME, target ACE I/D polymorphism DNA fragments (D = 190 bp and I = 490 bp) were identified within 25 s without loss of resolving power, which was ∼300 times faster than conventional SGE. In addition, PCR products of the ACE gene from human blood samples were detected after only 10 cycles by multi-channel PEFS-ME, but not by SGE. This parallel detection multichannel-based PEFS-ME method offers a powerful tool for fast high-throughput ACE I/D polymorphism screening with high sensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1028, 15 August 2016, Pages 77-85
نویسندگان
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