کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7626133 1494577 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a simple, rapid and high-throughput fluorescence polarization immunoassay for glycocholic acid in human urine
ترجمه فارسی عنوان
توسعه یک آزمایش ایمنولوژیک فلورسانس ساده، سریع و با کارایی بالا برای اسید گلیکوکولیک در ادرار انسان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this paper, a simple, rapid and high-throughput fluorescence polarization immunoassay (FPIA) based on polyclonal antibodies (PAb) is described for the determination of glycocholic acid (GCA) in human urine. Three fluorescein-labeled GCA (tracers) with different structures and spacer bridges were synthesized and purified by thin-layer chromatography (TLC). The structure effect of tracers on the assay was investigated and the sensitivity of best tracer in the optimized FPIA demonstrated an IC50 value of 306 ng/mL. The working range of FPIA was 36 ∼ 2 600 ng/mL and the limit of detection (LOD) was 9 ng/mL. The developed FPIA was time-saving that could be completed within 10 min. Human urine samples spiked with GCA were analyzed by this method, followed by confirmation with commercial enzyme immunoassay analysis (EIA). Excellent recoveries and correlation between these two methods were observed (R2 = 0.996), suggesting the developed FPIA could be applied to screening of GCA in human urine samples without complicated cleanup.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 158, 5 September 2018, Pages 431-437
نویسندگان
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