The analysis of phenolic compounds from the aerial parts of Eupatorium cannabinum L. subsp. cannabinum

Thirteen compounds were isolated and identified from the aerial parts of Eupatorium cannabinum subsp. cannabinum comprising six caffeic acid derivatives, five flavonol glycosides and two flavone aglycones. Additionally, the first HPLC-DAD-MS method for the phytochemical screening of phenolics in the analyzed plant material was developed allowing unequivocal identification of further six constituents occurring in the raw extract including four caffeic acid derivatives and two malonylated flavonol glycosides. This report is the first comprehensive study focused on phenolic compounds in the aerial parts of E. cannabinum. The chemotaxonomic significance in respect to the genus Eupatorium is discussed.