Antimicrobial activity of new materials based on the blends of collagen/chitosan/hyaluronic acid with gentamicin sulfate addition

In this study polymeric blends based on collagen, chitosan and hyaluronic acid in the form of thin films with the addition of gentamicin sulfate were obtained. Surface morphology of films was evaluated based on atomic force microscopy images. Surface free energy was measured and its polar and dispersive components were calculated. Moreover, oxygen and water vapor permeability through the material were measured as well as the water content in materials was studied. Thermal stability was determined by differential scanning calorimetry. Microbiological tests were performed to evaluate the diffusion of the drug from matrixes. The results showed that thin films based on collagen, chitosan and hyaluronic acid enriched in gentamicin sulfate inhibit the growth of both, Gram negative bacteria (E. coli and P. aeruginosa) and Gram positive ones (S. aureus).