کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326895 | 1540196 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
l-Arginine and allopurinol supplementation attenuates inflammatory mediators in human osteoblasts-osteoarthritis cells
ترجمه فارسی عنوان
مکمل ال-آرژنین و آلوپورینول، واسطه های التهابی را در سلول های استئوبلاست انسانی- استئوآرتریت کاهش می دهد
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
This study investigated the synergistic effects of l-arginine and allopurinol on antioxidant and inflammatory mediators in human osteoblasts-osteoarthritis (HOb-OA) cells. The cells were treated with allopurinol (50-150â¯mg/kg bwt) and l-arginine (50-150â¯mg/kg bwt) for 72â¯h. Cell viability, catalase, superoxide dismutase (SOD), glutathione peroxidase (Gpx), reduced glutathione (GSH), lipid peroxidation, and the inflammatory markers interleukin 6 (IL-6), interleukin 1β (IL-1β), nuclear factor κB (NF-κB) and tumor necrosis factor alpha (TNF-α) were measured. The combined supplementation with allopurinol and l-arginine increased catalase, SOD, GSH, and Gpx, while it decreased lipid peroxidation, IL-6, IL-1β, and TNF-α. While TNF-α, IL-6, IL-1β, and NF-κB mRNA and protein expression were higher in control HOb-OA cells, the combined supplementation with allopurinol and l-arginine substantially reduced their expression in HOb-OA cells by >40%. In summary, combined supplementation with allopurinol and l-arginine might be very effective in osteoarthritis. A search for therapeutic agents that inhibit inflammation could help to prevent and manage osteoarthritis. However, further studies need to determine the biochemical and molecular mechanisms of these agents in osteoarthritis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 118, Part A, 15 October 2018, Pages 716-721
Journal: International Journal of Biological Macromolecules - Volume 118, Part A, 15 October 2018, Pages 716-721
نویسندگان
Jichao Li, Zeng Zhang, Xiaohan Huang,