کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8495162 | 1552859 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quality improvement of fish feed by using insulin-like growth factor 1 as a potential indicator of amino acid deficiency in yellowtail
ترجمه فارسی عنوان
بهبود کیفیت خوراک ماهی با استفاده از عامل رشد 1 انسولین مانند شاخص بالقوه کمبود اسید آمینه در زرد رنگ
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کلمات کلیدی
آمینو اسید، تغذیه ماهی، شاخص فاکتور رشد مثل انسولین 1، پرولین، زردآلو،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
چکیده انگلیسی
We developed a new method for improving the quality of fish feed, by using insulin-like growth factor 1 (IGF1) as a potential indicator of dietary amino acid deficiency in yellowtail (Seriola quinqueradiata). First, 7 dispensable amino acid solutions (alanine, asparagine, cysteine, glutamine, glycine, serine and proline) and 0.9% NaCl (control) solution were injected into the dorsal muscle of fish using a syringe. Twenty-four hours post-administration, white muscle tissue was collected from the fish opposite to the injection site and assayed for igf1 mRNA expression. Only fish injected with proline showed a significant increase in igf1 mRNA expression levels. Subsequently, a feeding trial was conducted with 3 different diets-the control diet; the control diet supplemented with 1% proline; and the control diets supplemented with 3% proline. Juvenile yellowtails were fed exclusively with 1 of the 3 diets until apparent satiation. The body weight and feed efficiency were significantly higher in fish fed with the 3% proline-supplemented diet than fish fed with the control diet. Further, after 3Â weeks, the expression levels of igf1 in white muscle and liver were significantly higher in fish fed with the 3% proline-supplemented diet than in fish fed with the control diet. Our results suggest that igf1 mRNA expression in the white muscle tissue of yellowtails serves as a reliable indicator of amino acid deficiency. Additionally, the results of our feeding trial suggest that proline is required for juvenile yellowtail growth. Moreover, white muscle igf1 may represent a valuable tool for improving the quality of fish feed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volumes 426â427, 20 April 2014, Pages 264-269
Journal: Aquaculture - Volumes 426â427, 20 April 2014, Pages 264-269
نویسندگان
H. Fukada, Y. Fujii, H. Matsumoto, N. Takahashi, N. Hosomi, K. Morioka, T. Masumoto,