Anaplasma infection prevalence in beef and dairy cattle in the south east region of Botswana

Infection of cattle by the tick-borne intra-erythrocytic bacteria of the genus Anaplasma occurs worldwide. Nevertheless, prevalence rates in specific regions are still required to inform cattle farming management decisions. A study was carried out to determine Anaplasma infection prevalence in beef and dairy cattle in the south east region of Botswana. Two methods were used: competitive inhibition enzyme-linked immune-sorbent assay (cELISA) and conventional polymerase chain reaction (PCR). A total of 429 cattle consisting 207 beef and 222 dairy animals were sampled and tested. The prevalence was 91% and 31% by cELISA and PCR, respectively. A Z test revealed a statistical difference between the point prevalence as determined by cELISA compared to PCR (p = 0). There was no statistical difference between the point prevalence of Anaplasma infection as determined by cELISA (p = 0.45) between beef and dairy cattle. But there was a significant difference (p = 0.001) between the animals by PCR with the prevalence in beef cattle nearly double that in dairy cattle. Individual herd prevalence ranged from 79% to 100% by cELISA, and 0 to 100% by PCR. Though not statistically significant sero-prevalence in both beef and dairy animals tended to be higher in urban/peri-urban areas compared to rural areas. The cELISA mean percentage inhibition (PI) for all cattle was found to be 58.6 (95% CI: 56.8-60.4). There was no statistically significant difference between the mean PI of sera from beef cattle (56.4 (95% CI: 54.1-58.7)) as compared to dairy cattle (60.7 (95%CI: 58.0-63.3)). However, there was a tendency towards statistical significance with beef animals having a lower PI value than dairy animals. Anaplasma infection was endemic in cattle in the south east region of Botswana with similar infection in beef and dairy animals. Further research should be done to identify the strains prevalent in the cattle herds.