کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8552934 | 1562279 | 2018 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biological effects of adipocytes in sulfur mustard induced toxicity
ترجمه فارسی عنوان
اثرات بیولوژیکی آدیپوسیت ها در سمیت ناشی از گوگرد
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کلمات کلیدی
NMPTNF-aMnSODPPARHCART-PCRCOX-2IL-6CCK-8PBSproliferator-activated receptorMMPGC–MS - GC-MSLC–MS/MS - LC-MS / MSAdipokine - آدپیکینAdipocyte - آدیپوسیتPOPs - آلایندههای آلی دیرپاPersistent organic pollutants - آلایندههای آلی دیرپا(پایدار)interleukin-6 - اینترلوکین ۶Adipose tissue - بافت چربیHigh-content analysis - تجزیه و تحلیل با محتوای بالاELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاSerum - سرمCytotoxicity - سمیت سلولیSulfur mustard - سولفور موستارد یا گاز خردلmanganese superoxide dismutase - سوپر اکسید دیسموتاز منگنزcyclooxygenase 2 - سیکلوکوکسیژناز 2cell counting kit-8 - شمارش سلول کیت 8Liquid chromatography-tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع دو طرفهtumor necrosis factor-a - عامل نکروز تومور- aPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایMitochondrial membrane potential - پتانسیل غشای میتوکندریGas Chromatography-Mass Spectrometry - گاز کروماتوگرافی-اسپکترومتری جرمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Sulphur mustard (2,2â²-dichloroethyl sulfide; SM) is a vesicant chemical warfare agent whose mechanism of acute or chronic action is not known with any certainty and to date there is no effective antidote. SM accumulation in adipose tissue (AT) has been originally verified in our previous study. To evaluate the biological effect caused by the presence of abundant SM in adipocyte and assess the biological role of AT in SM poisoning, in vitro and in vivo experiments were performed. High content analysis revealed multi-cytotoxicity in SM exposed cells in a time and dose dependent manner, and adipocytes showed a relative moderate damage compared with non-adipocytes. Cell co-culture model was established and revealed the adverse effect of SM-exposed adipocyte supernatant on the growth of co-cultured cells. The pathological changes in AT from 10Â mg/kg SM percutaneously exposed rats were checked and inflammation phenomena were observed. The mRNA and protein levels of inflammation-related adipokines secreted from AT in rats exposed to 1, 3 and 10Â mg/kg doses of SM were determined by reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assays. The expressions of proinflammatory and anti-inflammatory adipokines together promoted the inflammation development in the body. The positive correlations between AT and serum adipokine levels were explored, which demonstrated a substantial role of AT in systemic inflammation responding to SM exposure. Thus, AT is not only a target of SM but also a modulator in the SM toxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 393, 15 January 2018, Pages 140-149
Journal: Toxicology - Volume 393, 15 January 2018, Pages 140-149
نویسندگان
Hua Xu, Zhongcai Gao, Peng Wang, Bin Xu, Yajiao Zhang, Long Long, Cheng Zong, Lei Guo, Weijian Jiang, Qinong Ye, Lili Wang, Jianwei Xie,