کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8841498 | 1615022 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The glutamate clearance function of adipose stromal cells-derived astrocytes
ترجمه فارسی عنوان
عملکرد ترخیص گلوتامات آستروسیت های حاصل از سلول های استرومائی چربی
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کلمات کلیدی
PBSADSCsPVDFVGLUTsGLT-1GFAPEAATsMSODMSO - DMSOMTT - MTTAstrocytes - آستروسیتInduction - القاءphosphate buffer - بافر فسفاتVesicular glutamate transporters - حمل و نقل گلوتامات Vesicularexcitatory amino acid transporters - حمل کننده های اسید آمینه تحریک کنندهCNS - دستگاه عصبی مرکزیpolyvinylidene difluoride - دی فلوئورید پلی وینیلیدینDimethylsulfoxide - دیمتیل سولفواکسیدAdipose-derived stromal cells - سلول های استرومای حاصل از چربیAdipose derived stromal cells - سلول های استرومای مشتق شده از چربیcentral nervous system - سیستم عصبی مرکزیMethionine sulfoxide - سیفوکسید متیونینoptical density - چگالی نوریglutamate - گلوتاماتGlutamine synthetase - گلوتامین سنتتاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
ADSCs-derived astrocytes qualify the morphology, ultrastructure and membrane electrical potential, which are all unique to astrocytes. But whether they have the glutamate clearance function like mature astrocytes is under exploration. ADSCs were extracted, cultured and induced into astrocytes for 48â¯h, 7d, 14d and 21d in vitro. Inverted phase contrast microscope was used to observe the morphology of the cells in each group. Immunocytochemistry assay, immunofluorescence assay and Western blotting were used to detect the expression of GFAP, EAAT2 and GS of the cells in each group. The cells were cultured in glutamate solution for 1, 2, 3 and 4â¯h respectively before the solution collected. The glutamate concentration of the solution was detected using Glutamate Colorimetric Assay Hit. ADSCs-derived astrocytes expressed GFAP, EAAT2 and GS, all of which increased gradually and reached peak when induced for 14â¯days. In induction for 48â¯h, 7d and 14d groups, the extracellular glutamate concentration decreased gradually during the cells cultured in glutamate solution for 1, 2, 3 and 4â¯h, among which the decrease extent was most prominent in 14d group, while the extracellular glutamate concentration had no change in uninduction and induction for 21d group. ADSCs-derived astrocytes expressed EAAT2 and GS, meanwhile had the function of clearing glutamate, which was prominent when induced into astrocytes for 7-14â¯days.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 677, 11 June 2018, Pages 94-102
Journal: Neuroscience Letters - Volume 677, 11 June 2018, Pages 94-102
نویسندگان
Zanzan Cheng, Ya Ou, Lili Zhang, Pingshu Zhang, Xiaodong Yuan, Wei Peng, Shujuan Wang, Xuhong Zhu, Liping Zhang, Yan Meng,