کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8858130 | 1619287 | 2017 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reverse micellar partitioning of Bovine Serum Albumin with novel system
ترجمه فارسی عنوان
معکوس واکسن سرم گاو آلبومین با سیستم رمان
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
چکیده انگلیسی
To overcome the difficulties associated with the conventional extraction process like poor selective extraction of biomolecule and scale up of the process, the reverse micellar system consist of AOT/n-heptanol was considered to extract Bovine Serum Albumin (BSA) as a model biomolecule. The maximum forward extraction of BSA from aqueous phase to micelle phase was observed at AOT concentration 160â¯mM, aqueous phase pH value of 4, NaCl concentration 0.8â¯M and 95% back extraction of BSA from micelle phase to stripping phase was obtained at 1â¯M NaCl concentration with the pH of 7.5. HPLC analysis confirmed the stability of BSA during extraction. The size and water content of the reverse micelle was also reported. The obtained results emphasize the application of the AOT/n-heptanol reverse micellar system for the extraction of BSA and may be utilized for the selective extraction of similar hydrophilic proteins from the complex sources.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Resource-Efficient Technologies - Volume 3, Issue 4, December 2017, Pages 491-494
Journal: Resource-Efficient Technologies - Volume 3, Issue 4, December 2017, Pages 491-494
نویسندگان
Swapnali S. Pawar, I. Regupathi, B.D. Prasanna,