کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8880351 1624795 2018 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Green fluorescent protein as a visual selection marker for oil palm transformation
ترجمه فارسی عنوان
پروتئین فلورسنت سبز به عنوان یک مارکر انتخاب بصری برای تبدیل نخل
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
چکیده انگلیسی
The aim of the study was to regenerate transgenic oil palm expressing the gfp gene throughout the whole plant by transforming oil palm cultures with organelle-targeted and non-organelle targeted gfp genes. In this study, oil palm embryogenic calli and immature embryos were separately bombarded with different constructs carrying either organelle-targeted (mitochondria, plastid or endoplasmic reticulum) or non-organelle targeted gfp gene in combination with the herbicide Basta resistance (bar) gene for regenerating transgenic oil palm expressing gfp. Transformed embryogenic calli were successfully regenerated on media containing Basta but failed to express the gfp gene in the regenerated plants. On the other hand, the bombarded immature embryos were either directly germinated or regenerated into whole plantlets via callogenesis on medium containing Basta. The regenerated plantlets demonstrated gfp expression only as patches on some parts of the plants such as roots and leaves and not in the whole plant, contrary to what was expected. Even though the plantlets failed to show gfp gene expression in the whole plant, molecular analyses revealed the presence of bar and gfp transgenes in the transgenic plants. Based on results obtained from this study, it could be concluded that the failure was due to the inability of gfp gene to express in the regenerated transgenic plants. In order to overcome this failure, further interventions are proposed which include the use of stronger promoters, use of different gfp versions and finally to use alternative transformation methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 115, May 2018, Pages 134-145
نویسندگان
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