Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10040482 | Archives of Medical Research | 2005 | 6 Pages |
Abstract
Significant statistical differences between the use of traditional techniques and PCR-RFLP were not found at genus level, but there were important differences at species and biotype level. Biochemical tests identified correctly the actinomycete isolates as belonging to Nocardia genus, but at N. asteroides complex level were not able to discern among their different species. PCR-RFLP is a rapid, non-expensive, and reliable method that allows to discriminate the N. asteroides complex species, identifying biotypes related to invasive disease. Our results suggest that the hospital environment was not a contamination source.
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Authors
José Francisco Valenzuela-Tovar, Cudberto Contreras-Pérez, Héctor Shibayama-Hernández, Lorena Chávez-González, Carlos Arturo Vázquez-Chacón, Hiram Olivera-DÃaz,