Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10126815 | Forensic Science International | 2018 | 11 Pages |
Abstract
In some autopsy cases, there are unknown natural toxins that are suspected to cause serious damage to the person. However, without reference materials, it is almost impossible to identify the suspicious natural toxins by GC-MS or LC-MS. In this case, a man drank mushroom âliquor with a meal at his home. Seven hours later, he was transported to the emergency room, and 12Â hours later, he died. In the ingested mushroom-infused-liquor, there were pieces of mushroom that were estimated to be Podostroma cornu-damae (Hypocreaceae) based on their morphological characteristics. To identify the species, chemical component analysis was conducted using LC-QTOF-MS/MS. Monoisotopic mass, fragment ions, and isotope distributions were obtained from the LC-QTOF-MS/MS analysis. In addition, fragment ions and structure matching were tested for target compound confirmation. In this analysis, several toxic trichothecene-type mycotoxins were identified including roridin D, roridin E, roridin Q, satratoxin G, satratoxin H, satratoxin H 12â²-acetate, satratoxin H 13â²-acetate, satratoxin H 12â²,13â²-diacetate, and verrucarol. At autopsy, heart blood, peripheral blood, and the stomach contents were collected, and only satratoxin H was detected in these samples. This is the first finding of a trichothecene-type mycotoxin in a human biological sample from an expected case of P. cornu-damae intoxication. We demonstrated that LC-QTOF-MS/MS analysis was an effective method for mushroom intoxication cases in the absence of reference materials. Additionally, the experience, knowledge, and analytical methods we obtained in this study will be great assets for solving other cases of possible natural toxin intoxication.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sanggil Choe, Sangwhan In, Youngjoon Jeon, Hyeyoung Choi, Suncheun Kim,