Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10128086 | Carbohydrate Polymers | 2018 | 21 Pages |
Abstract
Eclipta prostrata has long been used as a medicinal herb in China. EAP20-1, a homogeneous polysaccharide with anti-complementary activity had been obtained from E. prostrate by using anion-exchange and size-exclusion chromatography. In this study, we found that EAP20-1 could inhibit in vitro lymphocyte proliferation stimulated by concanavalin-A or anti-CD3/anti-CD28 antibodies. Furthermore, in experimental autoimmune encephalomyelitis (EAE) mice, EAP20-1 treatment relieved the clinical symptoms, accompanied by reduced neuroinflammation and demyelination in spinal cords. Mechanistically, EAP20-1 reduced the mRNA expression of interleukin (IL)-17, IL-22, and RAR-related orphan receptor gamma t (RORγt) in the spleen; inhibited auto-reactive T cell proliferation and decreased the percentage of Th17 cells in response to myelin oligodendrocyte glycoprotein (MOG35-55) ex vivo. Moreover, EAP20-1 directly inhibited naïve CD4â+âT cells differentiate into Th17 cells in vitro. These results indicating EAP20-1 could benefit EAE through inhibiting Th17 cell differentiation and suggesting a therapeutic potential of EAP20-1 in MS.
Keywords
TGF-βEAEiNOSLFBqRT-PCRArabinoseGlcLuxol Fast BluePTXMOGPhorbol-12-myristate-13-acetateFBSConARORγtEclipta prostrataH&EPMAexperimental autoimmune encephalomyelitisinterleukintransforming growth factor-betaEnzyme-linked immunosorbent assayELISAARAfetal bovine serumTH cellsTh17 cellsT helper cellspertussis toxininducible nitric oxide synthasetumor necrosis factorTNF-αMethylprednisoloneMultiple sclerosisHematoxylin & Eosinquantitative real-time polymerase chain reactionPolysaccharideGalGalactoseGlucosemyelin oligodendrocyte glycoprotein
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Ning Li, Songshan Shi, Feifei Yang, Hongwei Wang, Juan Su, Fei Huang, Hui Wu, Xiaojun Wu, Shunchun Wang,