Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10137123 | Toxicon | 2018 | 7 Pages |
Abstract
The main aim of this research was to evaluate the toxicokinetic characteristics of fusarenon-X (FX) and its metabolite, nivalenol (NIV), in goats. The amounts of FX and NIV in post-mitochondrial (S-9), microsomal and cytosolic fractions of diverse tissues of the goat were also investigated. FX was intravenously (iv) or orally (po) administered to goats at dosages of 0.25 and 1â¯mg/kg bw, respectively. The concentrations of FX and NIV in plasma, feces and urine were quantified by liquid chromatography tandem-mass spectrometry (LC-ESI-MS/MS). The concentrations of FX in plasma were quantified up to 8â¯h with both routes of administration. A large amount of NIV (metabolite) was quantifiable in plasma, urine and feces after both administrations. The Cmax value of FX was 413.39â¯Â±â¯206.84â¯ng/ml after po administration. The elimination half-life values were 1.64â¯Â±â¯0.32â¯h and 4.69â¯Â±â¯1.25â¯h after iv and po administration, respectively. In vitro experiments showed that the conversion FX-to-NIV mainly occurs in the liver microsomal fraction. This is the first study that evaluates the fate and metabolism of FX in ruminant species.
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Authors
Wanchalerm Phruksawan, Saranya Poapolathep, Mario Giorgi, Kanjana Imsilp, Chainarong Sakulthaew, Helen Owen, Amnart Poapolathep,