Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10137144 | Toxicon | 2018 | 4 Pages |
Abstract
The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.
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Authors
Yoko Kato-Unoki, Yuki Takai, Masato Kinoshita, Toshitaka Mochizuki, Ryohei Tatsuno, Yohei Shimasaki, Yuji Oshima,