Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10140896 | Food Chemistry | 2019 | 9 Pages |
Abstract
With genetically modified (GM) food circulating on the market, a rapid transgenic food screening method is needed to protect consumer rights. The on-site screening efficiency of GM food testing is low. We report rapid sample-to-answer detection of GM papayas with loop-mediated isothermal amplification (LAMP) and a compact, portable, integrated microfluidic platform using microfluidic lab-on-a-disc (LOAD). GM samples were differentiated from non-GM papaya, based on the detection of a specific GM (P-35S (Cauliflower mosaic virus 35S promoter)) and non-GM DNA marker (papain) in 15â¯min. The detection limits for DNA and juice from papaya were 10â¯pg/µL and 0.02â¯ÂµL, respectively. Our LOAD platform is a simple and robust solution for GM screening, which is anticipated to be a foundation for on-site testing of transgenic food.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jacky Fong-Chuen Loo, Grace Wing-Chiu But, Ho-Chin Kwok, Pui-Man Lau, Siu-Kai Kong, Ho-Pui Ho, Pang-Chui Shaw,