| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10140928 | Microchemical Journal | 2019 | 8 Pages |
Abstract
A simple and efficient functionalized graphene (f-graphene) doped chitosan (CS) based electrochemical aptasensor has been developed for ochratoxin A (OTA) detection. Use of f-graphene increased the electroactive surface area of the electrode and CS prevented leaching of the aptamer (APT) molecules. The dual properties of CS and f-graphene overall improved the sensor performance. The developed APT/SA/CS-f-graphene sensor has been characterized using Fourier Transform Infrared Spectroscopy (FTIR), Contact angle, Field emission - scanning electron microscopy (FE-SEM) and electrochemical studies. The aptasensor displayed OTA limit of detection (LOD) about 1â¯fg/mL for standard and 0.01â¯ng/mL for spiked sample within response time of 8â¯min. The reusable aptaelectrode retained 85% of initial current values after 7th reaction with stability of about 7â¯days. Besides, real application of the fabricated aptasensor has been evaluated in the grape juice samples. Recoveries of OTA in the range of 90-101% were estimated successfully.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Navpreet Kaur, Anu Bharti, Supriya Batra, Shilpa Rana, Shweta Rana, Aman Bhalla, Nirmal Prabhakar,