Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10146467 | Bioresource Technology | 2018 | 37 Pages |
Abstract
This work investigated sucrose metabolism in C. saccharoperbutylacetonicum. Inactivation of sucrose catabolism operon resulted in 28.9% decrease in sucrose consumption and 44.1% decrease in ABE production with sucrose as sole carbon source. Interestingly, a large amount of colloid-like polysaccharides were generated in the mutant, which might be due to inefficient intracellular sucrose metabolism. Deletion of transcriptional repressor gene successfully alleviated CCR and enhanced ABE production by 24.7%. Additional overexpression of endogenous sucrose pathway further elevated sucrose consumption and enhanced ABE production by 17.2%, 45.7%, or 22.5% compared to wild type with sucrose, mixed sugars or sugarcane juice as substrate, respectively. The engineered strain could be a robust platform for efficient biofuel production from inexpensive sucrose-based carbon sources.
Keywords
Related Topics
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Process Chemistry and Technology
Authors
Jie Zhang, Pixiang Wang, Xiaofei Wang, Jun Feng, Hardev S. Sandhu, Yi Wang,