Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10146982 | Biosensors and Bioelectronics | 2018 | 24 Pages |
Abstract
We describe a 3-dimensional scaffold nanocomposite oxidase nanozyme (termed PtNPs@MnO2) formed from 2-dimensional MnO2 nanosheets grown on the surface of large platinum nanoparticles (PtNPs). The two components act synergistically in the catalytic oxidation of 3,3â²,5,5â²-tetramethylbenzidine (TMB), due to the intimate contact between the two components and mutual electron transfer phenomena. The Wonton-like 3-dimensional structure of the nanocomposite is highly stable and was characterized by cryogenic electron microscopy (cryo-EM). The PtNPs@MnO2 nanocomposite was employed in a sensing assay for the determination of glutathione (GSH) and dopamine (DA) with a linear range of 0.2â¯Î¼M-11â¯Î¼M for GSH and 0.1â¯Î¼M-1.1â¯Î¼M for DA. The limit of detection (LOD) and limit of quantification (LOQ) of GSH are 0.05â¯Î¼M (S/Nâ¯=â¯3) and 0.17â¯Î¼M (S/Nâ¯=â¯10), respectively. The LOD and LOQ for DA were estimated as 0.025â¯Î¼M and 0.083â¯Î¼M. The method was applied to analyze GSH in human serum samples and DA in pharmaceuticals.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Jing Liu, Lingjie Meng, Zhaofu Fei, Paul J. Dyson, Lei Zhang,