Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10153071 | Biosensors and Bioelectronics | 2018 | 33 Pages |
Abstract
As cardiovascular diseases (CVD) are responsible for millions of deaths annually, there is a need for rapid and sensitive diagnosis of CVD at earlier stages. Aptamers generated by systematic evolution of ligands by exponential enrichment (SELEX) processes have been shown to be superior to conventional antibody-based cardiac biomarker detection. However, SELEX is a complicated, lengthy procedure requiring multiple rounds of extraction/amplification and well-trained personnel. To circumvent such issue, we designed an automated, miniaturized SELEX platform for the screening of aptamers towards three protein biomarkers associated with CVDs: N-terminal pro-peptide of B-type natriuretic peptide, human cardiac troponin I, and fibrinogen. The developed microfluidic platform was equipped with microfluidic devices capable of sample transport and mixing along with an on-chip nucleic acid amplification module such that the entire screening process (5 rounds of selection in 8â¯h.) could be performed consecutively on a single chip while consuming only 35â¯ÂµL of reagents in each cycle. This system may therefore serve as a promising, sensitive, cost-effective platform for the selection of aptamers specific for CVD biomarkers.
Keywords
CNCpolydimethysiloxanetris-acetate-EDTAPluronic P123P123EMVdeoxyribonucleotideTRISNT-proBNPdNTPNIHEtBrSELEXPBSPDMSdsDNAE. coliBSACVDsdouble-stranded DNAbovine serum albuminethidium bromideEscherichia coliCapillary electrophoresisCardiovascular diseasesTris(hydroxymethyl)aminomethaneSurface plasmon resonanceSPRTAEsystematic evolution of ligands by exponential enrichmentDissociation constantsRoom temperatureNational Institutes of HealthPhosphate-buffered salinebase-pairsPMMAPoly (methyl methacrylate)Creatine kinaseDe-ionized
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Anirban Sinha, Priya Gopinathan, Yi-Da Chung, Hsin-Ying Lin, Kuang-Hsien Li, Hsi-Pin Ma, Po-Chiun Huang, Shu-Chu Shiesh, Gwo-Bin Lee,