Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10154464 | Journal of Chromatography A | 2018 | 35 Pages |
Abstract
In gradient elution mode, the columns' order has a role (non-symmetrical system). When the last column has high enough efficiency, the gradient band compression effect may outperform the competing band broadening caused by dispersive and diffusive processes (peak sharpening). Therefore, in gradient mode, the columns should generally be sequentially placed according to their increasing efficiency.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Szabolcs Fekete, Santiago Codesido, Serge Rudaz, Davy Guillarme, Krisztián Horváth,