Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10156147 | Applied Clay Science | 2018 | 7 Pages |
Abstract
Bentonite (Bent) clay is a component of soil with useful properties for enzyme adsorption. In this work, the previously characterized antioxidant superoxide dismutase (SOD) enzyme named wGLP by wheat germin-like protein was immobilized in raw (Bent) and modified bentonites (Bent-DDA). The physicochemical characterizations of both supports were carried out. Successful adsorption of wGLP onto Bent and Bent-DDA occurred on the surface and within the interlayer spacing as was revealed by X-Ray Diffraction (XRD) and Thermogravimetric Analysis (TGA). The release of wGLP at different times and pHs was tested. While wGLP remained almost totally immobilized into Bent-DDA up to 96â¯h, it was released from Bent to reach nearly 60% after 72â¯h at pHâ¯7.5 and preserving its SOD activity. Since tomato cell viability under the presence of Bent-wGLP was maintained, Bent-wGLP complexes are potential carriers of antioxidants in tomato cell suspension cultures.
Related Topics
Physical Sciences and Engineering
Earth and Planetary Sciences
Geochemistry and Petrology
Authors
Andrea Y. Mansilla, Matias Lanfranconi, Vera A. Alvarez, Claudia A. Casalongué,